2sob
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(New page: 200px<br /><applet load="2sob" size="450" color="white" frame="true" align="right" spinBox="true" caption="2sob" /> '''SN-OB, OB-FOLD SUB-DOMAIN OF STAPHYLOCOCCAL ...)
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Revision as of 11:55, 21 November 2007
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SN-OB, OB-FOLD SUB-DOMAIN OF STAPHYLOCOCCAL NUCLEASE, NMR, 10 STRUCTURES
Overview
Similar folds often occur in proteins with dissimilar sequences. The, OB-fold forms a part of the structures of at least seven non-homologous, proteins that share either oligonucleotide or oligosaccharide binding, functions. A 1-103 fragment corresponding to the OB-fold of the 149 amino, acid residue staphylococcal nuclease gives NMR spectra characteristic of, an unfolded protein, i.e. the wild-type nuclease sequence is insufficient, to maintain a stable tertiary structure in the absence of the C-terminal, one-third of this single-domain protein. By contrast, the 1-103 fragment, of nuclease with the mutations Val66Leu and Gly88Val adopts a stable, tertiary structure. The NMR solution structure of this latter fragment is, a close variation of the OB-fold found in the X-ray structure of the, parent protein. The Val66Leu and Gly88Val mutations appear to stabilize, tertiary structure by consolidating the hydrophobic core of the nuclease, OB-fold sub-domain. Taken together, these results suggest that recurrent, structural motifs such as the OB-fold may in some cases represent vestiges, of autonomous folding units that, during evolution, have become integrated, into more complex cooperative folding domains.
About this Structure
2SOB is a Single protein structure of sequence from Staphylococcus aureus. This structure superseeds the now removed PDB entry 1SOB. Active as Micrococcal nuclease, with EC number 3.1.31.1 Full crystallographic information is available from OCA.
Reference
NMR structure of a stable "OB-fold" sub-domain isolated from staphylococcal nuclease., Alexandrescu AT, Gittis AG, Abeygunawardana C, Shortle D, J Mol Biol. 1995 Jul 7;250(2):134-43. PMID:7608966
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