2udp

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spinqualitylabelsall models 2udp, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

UDP-GALACTOSE 4-EPIMERASE COMPLEXED WITH UDP-PHENOL

Overview

UDP-galactose 4-epimerase from Escherichia coli catalyzes the, interconversion of UDP-glucose and UDP-galactose. In recent years, the, enzyme has been the subject of intensive investigation due in part to its, ability to facilitate nonstereospecific hydride transfer between beta-NADH, and a 4-keto hexopyranose intermediate. The first molecular model of the, epimerase from E. coli was solved to 2.5 A resolution with crystals grown, in the presence of a substrate analogue, UDP-phenol (Bauer AJ, Rayment I, Frey PA, Holden HM, 1992, Proteins Struct Funct Genet 12:372-381). There, were concerns at the time that the inhibitor did not adequately mimic the, sugar moiety of a true substrate. Here we describe the high-resolution, X-ray crystal structure of the ternary complex of UDP-galactose, 4-epimerase with NADH and UDP-phenol. The model was refined to 1.8 A, resolution with a final overall R-factor of 18.6%. This high-resolution, structural analysis demonstrates that the original concerns were unfounded, and that, in fact, UDP-phenol and UDP-glucose bind similarly. The, carboxamide groups of the dinucleotides, in both subunits, are displaced, significantly from the planes of the nicotinamide rings by hydrogen, bonding interactions with Ser 124 and Tyr 149. UDP-galactose 4-epimerase, belongs to a family of enzymes known as the short-chain dehydrogenases, which contain a characteristic Tyr-Lys couple thought to be important for, catalysis. The epimerase/NADH/UDP-phenol model presented here represents a, well-defined ternary complex for this family of proteins and, as such, provides important information regarding the possible role of the Tyr-Lys, couple in the reaction mechanism.

About this Structure

2UDP is a Single protein structure of sequence from Escherichia coli with NA, NAD, UPP and EDO as ligands. This structure superseeds the now removed PDB entry 1UDP. Active as UDP-glucose 4-epimerase, with EC number 5.1.3.2 Full crystallographic information is available from OCA.

Reference

High-resolution X-ray structure of UDP-galactose 4-epimerase complexed with UDP-phenol., Thoden JB, Frey PA, Holden HM, Protein Sci. 1996 Nov;5(11):2149-61. PMID:8931134

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