This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1r3g
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1r3g" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r3g, resolution 1.16Å" /> '''1.16A X-ray structur...)
Next diff →
Revision as of 19:55, 24 November 2007
|
1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues
Overview
[structure: see text] Oligonucleotides with a novel, 2'-O-[2-(guanidinium)ethyl] (2'-O-GE) modification have been synthesized, using a novel protecting group strategy for the guanidinium group. This, modification enhances the binding affinity of oligonucleotides to RNA as, well as duplex DNA (DeltaT(m) 3.2 degrees C per modification). The 2'-O-GE, modified oligonucleotides exhibited exceptional resistance to nuclease, degradation. The crystal structure of a palindromic duplex formed by a DNA, oligonucleotide with a single 2'-O-GE modification was solved at 1.16 A, resolution.
About this Structure
1R3G is a Protein complex structure of sequences from [1] with MG as ligand. Full crystallographic information is available from OCA.
Reference
2'-O-[2-(guanidinium)ethyl]-modified oligonucleotides: stabilizing effect on duplex and triplex structures., Prakash TP, Puschl A, Lesnik E, Mohan V, Tereshko V, Egli M, Manoharan M, Org Lett. 2004 Jun 10;6(12):1971-4. PMID:15176796
Page seeded by OCA on Sat Nov 24 22:02:37 2007
Categories: Protein complex | Egli, M. | Lesnik, E. | Manoharan, M. | Prakash, T.P. | Puschl, A. | Tereshko, V. | MG | Dna
