178d
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(New page: 200px<br /><applet load="178d" size="450" color="white" frame="true" align="right" spinBox="true" caption="178d, resolution 2.500Å" /> '''CRYSTAL STRUCTURE O...)
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Revision as of 19:59, 24 November 2007
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CRYSTAL STRUCTURE OF A DNA DUPLEX CONTAINING 8-HYDROXYDEOXYGUANINE.ADENINE BASE-PAIRS
Overview
The crystal structure of the oligonucleotide d(CGCAAATTO8GGCG), containing, the chemically modified base 8-hydroxydeoxyguanine (O8G), has been, determined at 2.5-A resolution and refined to a crystallographic R-factor, of 16.8%. The B-type DNA helix contains standard Watson-Crick base pairs, except at the mismatch sites, where O8G adopts a syn conformation and, forms hydrogen bonds to adenine in the anti conformation. The, thermodynamic stability of the duplex was found by UV melting techniques, to be intermediate between the native oligonucleotide d(CGCAAATTTGCG) and, an oligonucleotide containing A.G mispairs d(CGCAAATTGGCG). Comparison of, the structure of the O8G(syn).A(anti) base pair with those of Watson-Crick, base pairs has given a reason why O8G.A base pairs are not well repaired, by DNA proofreading enzymes.
About this Structure
178D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of a DNA duplex containing 8-hydroxydeoxyguanine-adenine base pairs., McAuley-Hecht KE, Leonard GA, Gibson NJ, Thomson JB, Watson WP, Hunter WN, Brown T, Biochemistry. 1994 Aug 30;33(34):10266-70. PMID:8068665
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