1n1o
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(New page: 200px<br /><applet load="1n1o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n1o, resolution 1.20Å" /> '''Crystal Structure of...)
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Revision as of 20:16, 24 November 2007
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Crystal Structure of a B-form DNA Duplex Containing (L)-alpha-threofuranosyl (3'-2') Nucleosides: A Four-Carbon Sugar is Easily Accommodated into the Backbone of DNA
Overview
(L)-alpha-Threofuranosyl-(3'-->2')-oligonucleotides (TNA) containing, vicinally connected phosphodiester linkages undergo informational base, pairing in an antiparallel strand orientation and are capable of, cross-pairing with RNA and DNA. TNA is derived from a sugar containing, only four carbon atoms and is one of the simplest potentially natural, nucleic acid alternatives investigated thus far in the context of a, chemical etiology of nucleic acid structure. Compared to DNA and RNA that, contain six covalent bonds per repeating nucleotide unit, TNA contains, only five. We have determined the atomic-resolution crystal structure of, the B-form DNA duplex [d(CGCGAA)Td(TCGCG)](2) containing a single, (L)-alpha-threofuranosyl thymine (T) per strand. In the modified duplex, base stacking interactions are practically unchanged relative to the, reference DNA structure. The orientations of the backbone at the TNA, incorporation sites are slightly altered in order to accommodate fewer, atoms and covalent bonds. The conformation of the threose is C4'-exo with, the 2'- and 3'-substituents assuming quasi-diaxial orientation.
About this Structure
1N1O is a Protein complex structure of sequences from [1] with MG as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of a B-form DNA duplex containing (L)-alpha-threofuranosyl (3'-->2') nucleosides: a four-carbon sugar is easily accommodated into the backbone of DNA., Wilds CJ, Wawrzak Z, Krishnamurthy R, Eschenmoser A, Egli M, J Am Chem Soc. 2002 Nov 20;124(46):13716-21. PMID:12431101
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