1n53

From Proteopedia

---

Revision as of 20:27, 24 November 2007

proteopedia linkproteopedia link

spinqualitylabelsall models 1n53 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

SOLUTION STRUCTURE OF B. SUBTILIS T BOX ANTITERMINATOR RNA

Overview

The T-box transcription antitermination regulatory system is an important, mechanism for regulation of expression of aminoacyl-tRNA synthetase, amino, acid biosynthesis and transporter gene expression in Gram-positive, bacteria. Antitermination is dependent on a complex set of interactions, between uncharged tRNA and the leader region of the mRNA of the regulated, gene. Here, we report the solution structure of a model RNA, based on the, Bacillus subtilis tyrS antiterminator, determined to an rmsd of 3.47A for, all nine converged structures and 2.66A for the seven structures, representing the consensus family. The antiterminator is comprised of two, short helices with an intervening 7nt bulge. The bulge region of the, antiterminator, which ultimately interacts with the acceptor end of tRNA, exhibits extensive stacking at the 3' end (encompassing the highly, conserved ACC residues) and is the site of a pronounced kink between the, two flanking helices. The 5' end of the bulge exhibits evidence of, conformational flexibility. On the basis of the structural studies, there, is no indication that the bases at the 5' end of the bulge that ultimately, base-pair with tRNA are pre-organized for binding. Instead, the data are, consistent with a model in which the stacking-induced structure at the 3', end of the bulge may facilitate the pre-selection of a set of, conformations for the tRNA to sample during binding.

About this Structure

1N53 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Solution structure of the Bacillus subtilis T-box antiterminator RNA: seven nucleotide bulge characterized by stacking and flexibility., Gerdeman MS, Henkin TM, Hines JV, J Mol Biol. 2003 Feb 7;326(1):189-201. PMID:12547201

Page seeded by OCA on Sat Nov 24 22:34:38 2007