1iuk
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1iuk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1iuk, resolution 1.70Å" /> '''The structure of nat...)
Next diff →
Revision as of 20:27, 24 November 2007
|
The structure of native ID.343 from Thermus thermophilus
Overview
TT1466 is a hypothetical protein from the extremely thermophilic bacterium, Thermus thermophilus HB8 and is highly conserved in bacteria and archaea., The selenomethionyl protein was synthesized by a cell-free system and the, crystal structure was determined at 2.0 A by MAD phasing. A native crystal, was used for structure refinement to 1.7 A. The structure is highly, homologous to that of the CoA-binding domain of the succinyl-CoA, synthetase from Escherichia coli, despite the protein having only 14%, sequence identity to this domain. An isothermal titration calorimetry, experiment was performed to investigate whether TT1466 binds CoA and, revealed high-affinity CoA binding of TT1466.
About this Structure
1IUK is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Structure of a conserved CoA-binding protein synthesized by a cell-free system., Wada T, Shirouzu M, Terada T, Ishizuka Y, Matsuda T, Kigawa T, Kuramitsu S, Park SY, Tame JR, Yokoyama S, Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1213-8. Epub 2003, Jun 27. PMID:12832765
Page seeded by OCA on Sat Nov 24 22:34:43 2007
