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1wcm

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(New page: 200px<br /> <applet load="1wcm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wcm, resolution 3.8&Aring;" /> '''COMPLETE 12-SUBUNIT ...)
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==About this Structure==
==About this Structure==
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1WCM is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] and [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with ZN and MG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WCM OCA]].
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1WCM is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] and [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with ZN and MG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Transferase Transferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WCM OCA]].
==Reference==
==Reference==
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[[Category: zinc-finger]]
[[Category: zinc-finger]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 15:55:42 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:25:57 2007''

Revision as of 06:21, 30 October 2007


1wcm, resolution 3.8Å

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COMPLETE 12-SUBUNIT RNA POLYMERASE II AT 3.8 ANG

Overview

We determined the x-ray structure of the RNA polymerase (Pol) II, subcomplex Rpb4/7 at 2.3 A resolution, combined it with a previous, structure of the 10-subunit polymerase core, and refined an atomic model, of the complete 12-subunit Pol II at 3.8-A resolution. Comparison of the, complete Pol II structure with structures of the Pol II core and free, Rpb4/7 shows that the core-Rpb4/7 interaction goes along with formation of, an alpha-helix in the linker region of the largest Pol II subunit and with, folding of the conserved Rpb7 tip loop. Details of the core-Rpb4/7, interface explain facilitated Rpb4/7 dissociation in a, temperature-sensitive Pol II mutant and specific assembly of Pol I with, its Rpb4/7 counterpart, A43/14. The refined atomic model of Pol II serves, as the new reference ... [(full description)]

About this Structure

1WCM is a [Protein complex] structure of sequences from [Saccharomyces cerevisiae] and [Saccharomyces cerevisiae] with ZN and MG as [ligands]. Active as [Transferase], with EC number [2.7.7.6]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Structures of complete RNA polymerase II and its subcomplex, Rpb4/7., Armache KJ, Mitterweger S, Meinhart A, Cramer P, J Biol Chem. 2005 Feb 25;280(8):7131-4. Epub 2004 Dec 9. PMID:15591044

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