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1wch

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(New page: 200px<br /> <applet load="1wch" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wch, resolution 1.85&Aring;" /> '''CRYSTAL STRUCTURE O...)
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==About this Structure==
==About this Structure==
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1WCH is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with PO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WCH OCA]].
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1WCH is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with PO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Hydrolase Hydrolase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WCH OCA]].
==Reference==
==Reference==
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[[Category: tyrosine phosphatase]]
[[Category: tyrosine phosphatase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 15:55:50 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:26:01 2007''

Revision as of 06:21, 30 October 2007


1wch, resolution 1.85Å

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CRYSTAL STRUCTURE OF PTPL1 HUMAN TYROSINE PHOSPHATASE MUTATED IN COLORECTAL CANCER- EVIDENCE FOR A SECOND PHOSPHOTYROSINE SUBSTRATE RECOGNITION POCKET

Overview

Protein-tyrosine phosphatase-L1 (PTPL1, also known as FAP-1, PTP1E, PTP-BAS, and PTPN13) is mutated in a significant number of colorectal, tumors and may play a role in down-regulating signaling responses mediated, by phosphatidylinositol 3-kinase, although the precise substrates are as, yet unknown. In this study, we describe a 1.8 A resolution crystal, structure of a fully active fragment of PTPL1 encompassing the catalytic, domain. PTPL1 adopts the standard PTP fold, albeit with an unusually, positioned additional N-terminal helix, and shows an ordered phosphate in, the active site. Interestingly, a positively charged pocket is located, near the PTPL1 catalytic site, reminiscent of the second phosphotyrosine, binding site in PTP1B, which is required to dephosphorylate peptides, ... [(full description)]

About this Structure

1WCH is a [Single protein] structure of sequence from [Homo sapiens] with PO4 as [ligand]. Active as [Hydrolase], with EC number [3.1.3.48]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Crystal structure of the PTPL1/FAP-1 human tyrosine phosphatase mutated in colorectal cancer: evidence for a second phosphotyrosine substrate recognition pocket., Villa F, Deak M, Bloomberg GB, Alessi DR, van Aalten DM, J Biol Chem. 2005 Mar 4;280(9):8180-7. Epub 2004 Dec 20. PMID:15611135

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