1evo
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(New page: 200px<br /><applet load="1evo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1evo" /> '''NMR OBSERVATION OF A NOVEL C-TETRAD'''<br />...)
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Revision as of 20:46, 24 November 2007
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NMR OBSERVATION OF A NOVEL C-TETRAD
Overview
We report the NMR structure of the DNA sequence d-TGGGCGGT in Na(+), solutions at neutral pH, containing a repeat sequence from SV40 viral, genome. The structure is a novel quadruplex incorporating the C-tetrad, formed by symmetrical pairing of four Cs via NH(2)&bond;O(2) H-bonds in a, plane. The C-tetrad has a wider cavity compared to G-tetrads and stacks, well over the adjacent G4-tetrad, but poorly on the G6 tetrad. The, quadruplex helix is largely underwound by 8-10 degrees compared to B-DNA, except at the C5-G6 step. To our knowledge this is the first report of, C-tetrad formation in DNA structures, and would be of significance from, the point of view of both structural diversity and specific recognition.
About this Structure
1EVO is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
NMR observation of a novel C-tetrad in the structure of the SV40 repeat sequence GGGCGG., Patel PK, Bhavesh NS, Hosur RV, Biochem Biophys Res Commun. 2000 Apr 21;270(3):967-71. PMID:10772934
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