1rsb
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(New page: 200px<br /><applet load="1rsb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rsb, resolution 2.17Å" /> '''X-ray study of the D...)
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Revision as of 21:03, 24 November 2007
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X-ray study of the DNA oligomer d(ATATAT) in P65 space group
Overview
We present and analyze the structure of the oligonucleotide d(ATATAT), found in two different forms by X-ray crystallography and in solution by, NMR. We find that in both crystal lattices the oligonucleotide forms an, antiparallel double helical duplex in which base pairing is of the, Hoogsteen type. The double helix is apparently very similar to the, standard B-form of DNA, with about 10 base pairs per turn. However, the, adenines in the duplex are flipped over; as a result, the physicochemical, features of both grooves of the helix are changed. In particular, the, minor groove is narrow and hydrophobic. On the other hand, d(ATATAT), displays a propensity to adopt the B conformation in solution. These, results confirm the polymorphism of AT-rich sequences in DNA. Furthermore, we show that extrahelical adenines and thymines can be minor groove, binders in Hoogsteen DNA.
About this Structure
1RSB is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
X-ray and NMR studies of the DNA oligomer d(ATATAT): Hoogsteen base pairing in duplex DNA., Abrescia NG, Gonzalez C, Gouyette C, Subirana JA, Biochemistry. 2004 Apr 13;43(14):4092-100. PMID:15065851
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