1f2n
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(New page: 200px<br /><applet load="1f2n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f2n, resolution 2.8Å" /> '''RICE YELLOW MOTTLE VI...)
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Revision as of 21:04, 24 November 2007
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RICE YELLOW MOTTLE VIRUS
Overview
BACKGROUND: Rice yellow mottle virus (RYMV) is a major pathogen that, dramatically reduces rice production in many African countries. RYMV, belongs to the genus sobemovirus, one group of plant viruses with, icosahedral capsids and single-stranded, positive-sense RNA genomes., RESULTS: The structure of RYMV was determined and refined to 2.8 A, resolution by X-ray crystallography. The capsid contains 180 copies of the, coat protein subunit arranged with T = 3 icosahedral symmetry. Each, subunit adopts a jelly-roll beta sandwich fold. The RYMV capsid structure, is similar to those of other sobemoviruses. When compared with these, viruses, however, the betaA arm of the RYMV C subunit, which is a, molecular switch that regulates quasi-equivalent subunit interactions, is, swapped with the 2-fold-related betaA arm to a similar, noncovalent, bonding environment. This exchange of identical structural elements across, a symmetry axis is categorized as 3D domain swapping and produces, long-range interactions throughout the icosahedral surface lattice., Biochemical analysis supports the notion that 3D domain swapping increases, the stability of RYMV. CONCLUSIONS: The quasi-equivalent interactions, between the RYMV proteins are regulated by the N-terminal ordered residues, of the betaA arm, which functions as a molecular switch. Comparative, analysis suggests that this molecular switch can also modulate the, stability of the viral capsids.
About this Structure
1F2N is a Single protein structure of sequence from Rice yellow mottle virus with CA as ligand. Full crystallographic information is available from OCA.
Reference
3D domain swapping modulates the stability of members of an icosahedral virus group., Qu C, Liljas L, Opalka N, Brugidou C, Yeager M, Beachy RN, Fauquet CM, Johnson JE, Lin T, Structure. 2000 Oct 15;8(10):1095-103. PMID:11080631
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