1j83
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(New page: 200px<br /><applet load="1j83" size="450" color="white" frame="true" align="right" spinBox="true" caption="1j83, resolution 1.70Å" /> '''STRUCTURE OF FAM17 C...)
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Revision as of 21:07, 24 November 2007
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STRUCTURE OF FAM17 CARBOHYDRATE BINDING MODULE FROM CLOSTRIDIUM CELLULOVORANS
Overview
The crystal structure of the Clostridium cellulovorans, carbohydrate-binding module (CBM) belonging to family 17 has been solved, to 1.7 A resolution by multiple anomalous dispersion methods. CBM17 binds, to non-crystalline cellulose and soluble beta-1,4-glucans, with a minimal, binding requirement of cellotriose and optimal affinity for cellohexaose., The crystal structure of CBM17 complexed with cellotetraose solved at 2.0, A resolution revealed that binding occurs in a cleft on the surface of the, molecule involving two tryptophan residues and several charged amino, acids. Thermodynamic binding studies and alanine scanning mutagenesis in, combination with the cellotetraose complex structure allowed the mapping, of the CBM17 binding cleft. In contrast to the binding groove, characteristic of family 4 CBMs, family 17 CBMs appear to have a very, shallow binding cleft that may be more accessible to cellulose chains in, non-crystalline cellulose than the deeper binding clefts of family 4 CBMs., The structural differences in these two modules may reflect, non-overlapping binding niches on cellulose surfaces.
About this Structure
1J83 is a Single protein structure of sequence from Clostridium cellulovorans with CA as ligand. Active as Cellulase, with EC number 3.2.1.4 Full crystallographic information is available from OCA.
Reference
Recognition of cello-oligosaccharides by a family 17 carbohydrate-binding module: an X-ray crystallographic, thermodynamic and mutagenic study., Notenboom V, Boraston AB, Chiu P, Freelove AC, Kilburn DG, Rose DR, J Mol Biol. 2001 Dec 7;314(4):797-806. PMID:11733998
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