1ato
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(New page: 200px<br /><applet load="1ato" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ato" /> '''THE STRUCTURE OF THE ISOLATED, CENTRAL HAIRP...)
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Revision as of 21:10, 24 November 2007
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THE STRUCTURE OF THE ISOLATED, CENTRAL HAIRPIN OF THE HDV ANTIGENOMIC RIBOZYME, NMR, 10 STRUCTURES
Overview
The structure of an RNA hairpin containing a seven-nucleotide loop that is, present in the self-cleaving sequence of hepatitis delta virus antigenomic, RNA was determined by high resolution NMR spectroscopy. The loop, which is, composed of only one purine and six pyrimidines, has a suprisingly stable, structure, mainly supported by sugar hydroxyl hydrogen bonds and base-base, and base-phosphate stacking interactions. Compared with the structurally, well-determined, seven-membered anticodon loop in tRNA, the sharp turn, which affects the required 180 degrees change in direction of the, sugar-phosphate backbone in the loop is shifted one nucleotide in the 3', direction. This change in direction can be characterized as a reversed, U-turn. It is expected that the reversed U-turn may be found frequently in, other molecules as well. There is evidence for a new non-Watson-Crick UC, base pair formed between the first and the last residue in the loop, while, most of the other bases in the loop are pointing outwards making them, accessible to solvent. From chemical modification, mutational and, photocrosslinking studies, a similar picture develops for the structure of, the hairpin in the active ribozyme indicating that the loop structure in, the isolated hairpin and in the ribozyme is very similar.
About this Structure
1ATO is a Protein complex structure of sequences from Hepatitis delta virus. Full crystallographic information is available from OCA.
Reference
The structure of the isolated, central hairpin of the HDV antigenomic ribozyme: novel structural features and similarity of the loop in the ribozyme and free in solution., Kolk MH, Heus HA, Hilbers CW, EMBO J. 1997 Jun 16;16(12):3685-92. PMID:9218809
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