1f5u
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(New page: 200px<br /><applet load="1f5u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f5u" /> '''SOLUTION STRUCTURE OF THE KISSING DIMER OF H...)
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Revision as of 21:12, 24 November 2007
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SOLUTION STRUCTURE OF THE KISSING DIMER OF H3 GACG STEM-LOOP IN THE 5'-END DIMERIZATION SIGNAL OF MOLONEY MURINE LEUKEMIA VIRUS GENOMIC RNA
Overview
The dimerization of viral RNA through noncovalent interactions at their 5', ends is a key step in the life cycle of retroviruses. In Moloney murine, leukemia virus, three stem-loops are important in this process. One is a, self-complementary tetraloop (H1), but the other two stem-loops (H2, H3), contain highly conserved GACG tetraloops that are not self-complementary, sequences. Using two-dimensional NMR, we determined the structure of the, H3 stem-loop. Surprisingly, it forms a stable, homodimeric kissing complex, through only two intermolecular G small middle dotC base pairs., Cross-strand interactions of the adenines adjacent to the intermolecular G, small middle dotC base pairs, plus unusual strong electrostatic, interactions around the base pairs, contribute to the unexpected, stability. This structure shows how even stem-loops without, self-complementary sequences can facilitate the intermolecular recognition, between two identical RNAs, and thus initiate dimerization and, encapsidation of retroviral RNAs.
About this Structure
1F5U is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A retroviral RNA kissing complex containing only two G.C base pairs., Kim CH, Tinoco I Jr, Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9396-401. PMID:10931958
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