1ryj

From Proteopedia

---

Revision as of 21:23, 24 November 2007

proteopedia linkproteopedia link

spinqualitylabelsall models 1ryj Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Solution NMR Structure of Protein Mth1743 from Methanobacterium thermoautotrophicum. Ontario Centre for Structural Proteomics target MTH1743_1_70; Northeast Structural Genomics Consortium Target TT526.

Overview

The influx of genomic sequence information has led to the concept of, structural proteomics, the determination of protein structures on a, genome-wide scale. Here we describe an approach to structural proteomics, of small proteins using NMR spectroscopy. Over 500 small proteins from, several organisms were cloned, expressed, purified, and evaluated by NMR., Although there was variability among proteomes, overall 20% of these, proteins were found to be readily amenable to NMR structure determination., NMR sample preparation was centralized in one facility, and a distributive, approach was used for NMR data collection and analysis. Twelve structures, are reported here as part of this approach, which allowed us to infer, putative functions for several conserved hypothetical proteins.

About this Structure

1RYJ is a Single protein structure of sequence from Methanothermococcus thermolithotrophicus. Full crystallographic information is available from OCA.

Reference

An NMR approach to structural proteomics., Yee A, Chang X, Pineda-Lucena A, Wu B, Semesi A, Le B, Ramelot T, Lee GM, Bhattacharyya S, Gutierrez P, Denisov A, Lee CH, Cort JR, Kozlov G, Liao J, Finak G, Chen L, Wishart D, Lee W, McIntosh LP, Gehring K, Kennedy MA, Edwards AM, Arrowsmith CH, Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1825-30. PMID:11854485

Page seeded by OCA on Sat Nov 24 23:31:11 2007