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1gk0
From Proteopedia
(New page: 200px<br /> <applet load="1gk0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gk0, resolution 2.50Å" /> '''STRUCTURE-BASED PRE...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1GK0 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]] with PO4 and EDO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GK0 OCA]]. | + | 1GK0 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]] with PO4 and EDO as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Hydrolase Hydrolase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11]]. Structure known Active Site: POB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GK0 OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: x-raz structure]] | [[Category: x-raz structure]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:28:59 2007'' |
Revision as of 06:24, 30 October 2007
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STRUCTURE-BASED PREDICTION OF MODIFICATIONS IN GLUTARYLAMIDASE TO ALLOW SINGLE-STEP ENZYMATIC PRODUCTION OF 7-AMINOCEPHALOSPORANIC ACID FROM CEPHALOSPORIN C
Overview
Glutarylamidase is an important enzyme employed in the commercial, production of 7-aminocephalosporanic acid, a starting compound in the, synthesis of cephalosporin antibiotics. 7-aminocephalosporanic acid is, obtained from cephalosporin C, a natural antibiotic, either chemically or, by a two-step enzymatic process utilizing the enzymes D-amino acid oxidase, and glutarylamidase. We have investigated possibilities for redesigning, glutarylamidase for the production of 7-aminocephalosporanic acid from, cephalosporin C in a single enzymatic step. These studies are based on the, structures of glutarylamidase, which we have solved with bound phosphate, and ethylene glycol to 2.5 A resolution and with bound glycerol to 2.4 A., The phosphate binds near the catalytic serine in a way that mimics ... [(full description)]
About this Structure
1GK0 is a [Protein complex] structure of sequences from [Pseudomonas sp.] with PO4 and EDO as [ligands]. Active as [Hydrolase], with EC number [3.5.1.11]. Structure known Active Site: POB. Full crystallographic information is available from [OCA].
Reference
Structure-based prediction of modifications in glutarylamidase to allow single-step enzymatic production of 7-aminocephalosporanic acid from cephalosporin C., Fritz-Wolf K, Koller KP, Lange G, Liesum A, Sauber K, Schreuder H, Aretz W, Kabsch W, Protein Sci. 2002 Jan;11(1):92-103. PMID:11742126
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