1jox
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(New page: 200px<br /><applet load="1jox" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jox" /> '''NMR Structure of the LP5.1 Hairpin from Baci...)
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Revision as of 21:52, 24 November 2007
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NMR Structure of the LP5.1 Hairpin from Bacillus RNase P RNA Refined with Residual Dipolar Couplings
Overview
Long-range interactions involving the P5.1 hairpin of Bacillus RNase P RNA, are thought to form a structural truss to support RNA folding and, activity. We determined the structure of this element by NMR and refined, the structure using residual dipolar couplings from a sample weakly, oriented in a dilute liquid crystalline mixture of polyethylene glycol and, hexanol. Dipolar coupling refinement improved the global precision of the, structure from 1.5 to 1.2 A (to the mean), revised the bend angle between, segments of the P5.1 stem and corroborated the structure of the loop, region. The UGAGAU hexaloop of P5.1 contains two stacks of bases on, opposite sides of the loop, distinguishing it from GNRA tetraloops. The, unusual conformation of the juxtaposed uracil residues within the hexaloop, may explain their requirement in transactivation assays.
About this Structure
1JOX is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of the UGAGAU hexaloop that braces Bacillus RNase P for action., Leeper TC, Martin MB, Kim H, Cox S, Semenchenko V, Schmidt FJ, Van Doren SR, Nat Struct Biol. 2002 May;9(5):397-403. PMID:11927952
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