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1wj9
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(New page: 200px<br /><applet load="1wj9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wj9, resolution 1.90Å" /> '''Crystal structure of...)
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Revision as of 21:55, 24 November 2007
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Crystal structure of a CRISPR-associated protein from thermus thermophilus
Overview
We have determined the crystal structure of hypothetical protein TTHB192, from Thermus thermophilus HB8 at 1.9 A resolution. This protein is a, member of the Escherichia coli ygcH sequence family, which contains, approximately 15 sequence homologs of bacterial origin. These homologs, have a high isoelectric point. The crystal structure reveals that TTHB192, consists of two independently folded domains, and that each domain, exhibits a ferredoxin-like fold with a four-stranded antiparallel, beta-sheet packed on one side by alpha-helices. These two tandem domains, face each other to generate a beta-sheet platform. TTHB192 displays, overall structural similarity to Sex-lethal protein and poly(A)-binding, protein fragments. These proteins have RNA binding activity which is, supported by a beta-sheet platform formed by two tandem repeats of an RNA, recognition motif domain with signature sequence motifs on the beta-sheet, surface. Although TTHB192 does not have the same signature sequence motif, as the RNA recognition motif domain, the presence of an evolutionarily, conserved basic patch on the beta-sheet platform could be functionally, relevant for nucleic acid-binding. This report shows that TTHB192 and its, sequence homologs adopt an RNA recognition motif-like domain and provides, the first testable functional hypothesis for this protein family.
About this Structure
1WJ9 is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Crystal structure of hypothetical protein TTHB192 from Thermus thermophilus HB8 reveals a new protein family with an RNA recognition motif-like domain., Ebihara A, Yao M, Masui R, Tanaka I, Yokoyama S, Kuramitsu S, Protein Sci. 2006 Jun;15(6):1494-9. Epub 2006 May 2. PMID:16672237
Page seeded by OCA on Sun Nov 25 00:02:48 2007
