1nzg
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(New page: 200px<br /><applet load="1nzg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nzg, resolution 1.60Å" /> '''Crystal structure of...)
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Revision as of 21:58, 24 November 2007
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Crystal structure of A-DNA decamer GCGTA(3ME)ACGC, with a modified 5-methyluridine
Overview
[structure: see text] Oligonucleotides with a novel, 2'-O-[2-[2-(N,N-dimethylamino)ethoxy]ethyl] (2'-O-DMAEOE) modification, have been synthesized. This modification, a cationic analogue of the, 2'-O-(2-methoxyethyl) (2'-O-MOE) modification, exhibits high binding, affinity to target RNA (but not to DNA) and exceptional resistance to, nuclease degradation. Analysis of the crystal structure of a, self-complementary oligonucleotide containing a single 2'-O-DMAEOE, modification explains the importance of charge factors and gauche effects, on the observed antisense properties. 2'-O-DMAEOE modified, oligonucleotides are ideal candidates for antisense drugs.
About this Structure
1NZG is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
2'-O-[2-[2-(N,N-dimethylamino)ethoxy]ethyl] modified oligonucleotides: symbiosis of charge interaction factors and stereoelectronic effects., Prhavc M, Prakash TP, Minasov G, Cook PD, Egli M, Manoharan M, Org Lett. 2003 Jun 12;5(12):2017-20. PMID:12790517
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