1s9h
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(New page: 200px<br /><applet load="1s9h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s9h, resolution 2.40Å" /> '''Crystal Structure of...)
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Revision as of 21:59, 24 November 2007
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Crystal Structure of Adeno-associated virus Type 2 Rep40
Overview
We report here the crystal structure of an SF3 DNA helicase, Rep40, from, adeno-associated virus 2 (AAV2). We show that AAV2 Rep40 is structurally, more similar to the AAA(+) class of cellular proteins than to DNA, helicases from other superfamilies. The structure delineates the expected, Walker A and B motifs, but also reveals an unexpected "arginine finger", that directly implies the requirement of Rep40 oligomerization for ATP, hydrolysis and helicase activity. Further, the Rep40 AAA(+) domain is, novel in that it is unimodular as opposed to bimodular. Altogether, the, structural connection to AAA(+) proteins defines the general architecture, of SF3 DNA helicases, a family that includes simian virus 40 (SV40) T, antigen, as well as provides a conceptual framework for understanding the, role of Rep proteins during AAV DNA replication, packaging, and, site-specific integration.
About this Structure
1S9H is a Single protein structure of sequence from Adeno-associated virus 2. Full crystallographic information is available from OCA.
Reference
Crystal structure of the SF3 helicase from adeno-associated virus type 2., James JA, Escalante CR, Yoon-Robarts M, Edwards TA, Linden RM, Aggarwal AK, Structure. 2003 Aug;11(8):1025-35. PMID:12906833
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