1s9l
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1s9l" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s9l" /> '''NMR Solution Structure of a Parallel LNA Qua...)
Next diff →
Revision as of 21:59, 24 November 2007
|
NMR Solution Structure of a Parallel LNA Quadruplex
Overview
The solution structure of a locked nucleic acid (LNA) quadruplex, formed, by the oligomer d(TGGGT), containing only conformationally restricted LNA, residues is reported. NMR and CD spectroscopy, as well as molecular, dynamics and mechanic calculations, has been used to characterize the, complex. The molecule adopts a parallel stranded conformation with a, 4-fold rotational symmetry, showing a right-handed helicity and the, guanine residues in an almost planar conformation with three well-defined, G-tetrads. The thermal stability of Q-LNA has been found to be comparable, with that of [r(UGGGU)]4, while a T(m) increment of 20 degrees C with, respect to the corresponding DNA quadruplex structure [d(TGGGT)]4 has been, observed. The structural features of the LNA quadruplex reported here may, open new perspectives for the biological application of LNAs as novel, versatile tools to design aptamer or catalyst oligonucleotides.
About this Structure
1S9L is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
NMR solution structure of a parallel LNA quadruplex., Randazzo A, Esposito V, Ohlenschlager O, Ramachandran R, Mayola L, Nucleic Acids Res. 2004 Jun 4;32(10):3083-92. Print 2004. PMID:15181173
Page seeded by OCA on Sun Nov 25 00:07:27 2007
