1wky

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spinqualitylabelsall models 1wky, resolution 1.65Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module

Overview

An alkaline mannanase (EC 3.2.1.78) from the alkaliphilic Bacillus sp., strain JAMB-602 was cloned and sequenced. The deduced amino-acid sequence, of the enzyme suggested that the enzyme consists of a catalytic and, unknown additional domains. The recombinant enzyme expressed by B., subtilis was crystallized using the hanging-drop vapour-diffusion method, at 277 K. X-ray diffraction data were collected to 1.65 A. The crystal, belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 70.7, b = 79.5, c = 80.4 A. The asymmetric unit contains one protein molecule, with a corresponding VM of 2.26 A3 Da(-1) and a solvent content of 45.6%., Molecular replacement for initial phasing was carried out using the, three-dimensional structure of a mannanase from Thermomonospora fusca as a, search model, which corresponds to the catalytic domain of the alkaline, mannanase. It gave sufficient phases to build the unknown domain.

About this Structure

1WKY is a Protein complex structure of sequences from Bacillus sp. with CL, CA and NA as ligands. Active as Mannan endo-1,4-beta-mannosidase, with EC number 3.2.1.78 Full crystallographic information is available from OCA.

Reference

Crystallization and preliminary X-ray study of alkaline mannanase from an alkaliphilic Bacillus isolate., Akita M, Takeda N, Hirasawa K, Sakai H, Kawamoto M, Yamamoto M, Grant WD, Hatada Y, Ito S, Horikoshi K, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1490-2. Epub 2004, Jul 21. PMID:15272186

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