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1s9s

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(New page: 200px<br /><applet load="1s9s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s9s" /> '''SOLUTION STRUCTURE OF MLV PSI SITE'''<br /> ...)
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Revision as of 22:01, 24 November 2007

Image:1s9s.gif

1s9s

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SOLUTION STRUCTURE OF MLV PSI SITE

Overview

The full length, positive-strand genome of the Moloney Murine Leukemia, Virus contains a "core encapsidation signal" that is essential for, efficient genome packaging during virus assembly. We have determined the, structure of a 101-nucleotide RNA that contains this signal (called mPsi), using a novel isotope-edited NMR approach. The method is robust and should, be generally applicable to larger RNAs. mPsi folds into three stem loops, two of which (SL-C and SL-D) co-stack to form an extended helix. The third, stem loop (SL-B) is connected to SL-C by a flexible, four-nucleotide, linker. The structure contains five mismatched base-pairs, an unusual C.CG, base-triple platform, and a novel "A-minor K-turn," in which unpaired, adenosine bases A340 and A341 of a GGAA bulge pack in the minor groove of, a proximal stem, and a bulged distal uridine (U319) forms a hydrogen bond, with the phosphodiester of A341. Phylogenetic analyses indicate that these, essential structural elements are conserved among the murine C-type, retroviruses.

About this Structure

1S9S is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

NMR structure of the 101-nucleotide core encapsidation signal of the Moloney murine leukemia virus., D'Souza V, Dey A, Habib D, Summers MF, J Mol Biol. 2004 Mar 19;337(2):427-42. PMID:15003457

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