1fqp
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1fqp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fqp" /> '''INTRAMOLECULAR QUADRUPLEX DNA WITH THREE GGG...)
Next diff →
Revision as of 22:06, 24 November 2007
|
INTRAMOLECULAR QUADRUPLEX DNA WITH THREE GGGG REPEATS, NMR, PH 6.7, 0.1 M NA+ AND 4 MM (STRAND CONCENTRATION), 5 STRUCTURES
Overview
The solution structure of the DNA quadruplex formed by the association of, two strands of the DNA oligonucleotide, d(G3T4G3), in NaCl solution has, been determined by 1H two-dimensional NMR techniques, full relaxation, matrix calculations and restrained molecular dynamics. The refined, structure incorporates the sequences 5'-G1sG2AG3AT4AT5AT6AT7AG8sG9AG10A-3', and 5'-G11sG12AG13AT14AT15AT16AT17AG18sG19sG20A-3' (where S and A denote, syn and anti, respectively) in a three-quartet, diagonal-looped structure, that we [Strahan, G. D., Shafer, R. H. & Keniry, M. A. (1994) Nucleic, Acids Res. 22, 5447-5455] and others [Smith, F. W., Lau, F. W. & Feigon, J. (1994) Proc. Natl. Acad. Sci. USA 91, 10546-10550] have described. The, loop structure is compact and incorporates many of the features found in, duplex hairpin loops including base stacking, intraloop hydrogen bonding, and extensive van der Waals' interactions. The first and third loop, thymines stack over the outermost G-quartet and are also associated by, hydrogen bonding. The second and the fourth loop thymines fold inwards in, order to enhance van der Waals' interactions. The unexpected sequential, syn-syn deoxyguanosines in the quadruplex stem appear to be a direct, consequence of the way DNA oligonucleotides fold and the subsequent search, for the most stable loop structure. The implications of loop sequence and, length on the structure of quadruplexes are discussed.
About this Structure
1FQP is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of the Na+ form of the dimeric guanine quadruplex [d(G3T4G3)]2., Keniry MA, Strahan GD, Owen EA, Shafer RH, Eur J Biochem. 1995 Oct 15;233(2):631-43. PMID:7588810
Page seeded by OCA on Sun Nov 25 00:13:45 2007
