1fsv
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(New page: 200px<br /><applet load="1fsv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fsv" /> '''FULL SEQUENCE DESIGN 1 (FSD-1) OF BETA BETA ...)
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Revision as of 22:11, 24 November 2007
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FULL SEQUENCE DESIGN 1 (FSD-1) OF BETA BETA ALPHA MOTIF, NMR, MINIMIZED AVERAGE STRUCTURE
Overview
The first fully automated design and experimental validation of a novel, sequence for an entire protein is described. A computational design, algorithm based on physical chemical potential functions and, stereochemical constraints was used to screen a combinatorial library of, 1.9 x 10(27) possible amino acid sequences for compatibility with the, design target, a betabetaalpha protein motif based on the polypeptide, backbone structure of a zinc finger domain. A BLAST search shows that the, designed sequence, full sequence design 1 (FSD-1), has very low identity, to any known protein sequence. The solution structure of FSD-1 was solved, by nuclear magnetic resonance spectroscopy and indicates that FSD-1 forms, a compact well-ordered structure, which is in excellent agreement with the, design target structure. This result demonstrates that computational, methods can perform the immense combinatorial search required for protein, design, and it suggests that an unbiased and quantitative algorithm can be, used in various structural contexts.
About this Structure
1FSV is a Protein complex structure of sequences from Synthetic construct. Full crystallographic information is available from OCA.
Reference
De novo protein design: fully automated sequence selection., Dahiyat BI, Mayo SL, Science. 1997 Oct 3;278(5335):82-7. PMID:9311930
Page seeded by OCA on Sun Nov 25 00:18:48 2007
