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1sfu
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(New page: 200px<br /><applet load="1sfu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sfu, resolution 2.0Å" /> '''Crystal structure of ...)
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Revision as of 22:17, 24 November 2007
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Crystal structure of the viral Zalpha domain bound to left-handed Z-DNA
Overview
A conserved feature of poxviruses is a protein, well characterized as E3L, in vaccinia virus, that confers IFN resistance on the virus. This protein, comprises two domains, an N-terminal Z-DNA-binding protein domain (Zalpha), and a C-terminal double-stranded RNA-binding domain. Both are required for, pathogenicity of vaccinia virus in mice infected by intracranial, injection. Here, we describe the crystal structure of the Zalpha domain, from the E3L-like protein of Yaba-like disease virus, a Yatapoxvirus, in a, complex with Z-DNA, solved at a 2.0-A resolution. The DNA contacting, surface of Yaba-like disease virus Zalpha(E3L) closely resembles that of, other structurally defined members of the Zalpha family, although some, variability exists in the beta-hairpin region. In contrast to the, Z-DNA-contacting surface, the nonbinding surface of members of the Zalpha, family are unrelated; this surface may effect protein-specific, interactions. The presence of the conserved and tailored Z-DNA-binding, surface, which interacts specifically with the zigzag backbone and syn, base diagnostic of the Z-form, reinforces the importance to poxvirus, infection of the ability of this protein to recognize the Z-conformation.
About this Structure
1SFU is a Single protein structure of sequence from Viruses. Full crystallographic information is available from OCA.
Reference
A poxvirus protein forms a complex with left-handed Z-DNA: crystal structure of a Yatapoxvirus Zalpha bound to DNA., Ha SC, Lokanath NK, Van Quyen D, Wu CA, Lowenhaupt K, Rich A, Kim YG, Kim KK, Proc Natl Acad Sci U S A. 2004 Oct 5;101(40):14367-72. Epub 2004 Sep 24. PMID:15448208
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