1jy6
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(New page: 200px<br /><applet load="1jy6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jy6" /> '''B4DIMERA: A DE NOVO DESIGNED FOUR-STRANDED B...)
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Revision as of 22:18, 24 November 2007
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B4DIMERA: A DE NOVO DESIGNED FOUR-STRANDED BETA-SHEET ASSEMBLED USING A DISULFIDE BOND
Overview
The design and characterization of an open eight-stranded beta-sheet in a, synthetic, 2-fold symmetric 70-residue peptide is described. The design, strategy involves the generation of a 35-residue four-stranded beta-sheet, peptide in which successive hairpins are nucleated by appropriately, positioned (D)Pro-Xxx sequences. Oxidative dimerization using a single Cys, residue positioned at the center of the C-terminal strand results in a, disulfide-bridged eight-stranded structure. Nuclear Overhauser effects, firmly establish an eight-stranded beta-sheet in methanol. In water, the, outer strands are frayed, but a well-defined four-stranded beta-sheet, stabilized by a disulfide bridge and a hydrophobic cluster is determined, from NMR data. Comparison of the precursor peptide with the, disulfide-bridged dimer reveals considerable enhancement of beta-sheet, content in the latter, suggesting that the disulfide cross-link is an, effective strategy for the stabilization of beta-sheets.
About this Structure
1JY6 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Design and construction of an open multistranded beta-sheet polypeptide stabilized by a disulfide bridge., Venkatraman J, Nagana Gowda GA, Balaram P, J Am Chem Soc. 2002 May 8;124(18):4987-94. PMID:11982362
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