1woq
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(New page: 200px<br /><applet load="1woq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1woq, resolution 1.80Å" /> '''Crystal Structure of...)
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Revision as of 22:19, 24 November 2007
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Crystal Structure of Inorganic Polyphosphate/ATP-Glucomannokinase From Arthrobacter sp. strain KM At 1.8 A Resolution
Overview
Inorganic polyphosphate (poly(P)) is a biological high energy compound, presumed to be an ancient energy carrier preceding ATP. Several, poly(P)-dependent kinases that use poly(P) as a phosphoryl donor are known, to function in bacteria, but crystal structures of these kinases have not, been solved. Here we present the crystal structure of bacterial, poly(P)/ATP-glucomannokinase, belonging to Gram-positive bacterial, glucokinase, complexed with 1 glucose molecule and 2 phosphate molecules, at 1.8 A resolution, being the first among poly(P)-dependent kinases and, bacterial glucokinases. The poly(P)/ATP-glucomannokinase structure enabled, us to understand the structural relationship of bacterial glucokinase to, eucaryotic hexokinase and ADP-glucokinase, which has remained a matter of, debate. These comparisons also enabled us to propose putative binding, sites for phosphoryl groups for ATP and especially for poly(P) and to, obtain insights into the evolution of kinase, particularly from primordial, poly(P)-specific to ubiquitous ATP-specific proteins.
About this Structure
1WOQ is a Single protein structure of sequence from Arthrobacter sp. with GLC and PO4 as ligands. Active as Polyphosphate--glucose phosphotransferase, with EC number 2.7.1.63 Full crystallographic information is available from OCA.
Reference
Crystal structure of bacterial inorganic polyphosphate/ATP-glucomannokinase. Insights into kinase evolution., Mukai T, Kawai S, Mori S, Mikami B, Murata K, J Biol Chem. 2004 Nov 26;279(48):50591-600. Epub 2004 Sep 17. PMID:15377666
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