1nbm
From Proteopedia
(New page: 200px<br /> <applet load="1nbm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nbm, resolution 3.0Å" /> '''THE STRUCTURE OF BOV...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1NBM is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with MG, PO4, ATP and ADP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.34 3.6.1.34]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NBM OCA]]. | + | 1NBM is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with MG, PO4, ATP and ADP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Hydrolase Hydrolase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.34 3.6.1.34]]. Structure known Active Sites: CA1, CA2, CA3, PL1, PL2, PL3, PL4, PL5, PL6 and PL7. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NBM OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: inhibition]] | [[Category: inhibition]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:32:39 2007'' |
Revision as of 06:27, 30 October 2007
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THE STRUCTURE OF BOVINE F1-ATPASE COVALENTLY INHIBITED WITH 4-CHLORO-7-NITROBENZOFURAZAN
Overview
BACKGROUND: F1-ATPase is the globular domain of F1F0-ATP synthase that, catalyses the hydrolysis of ATP to ADP and phosphate. The crystal, structure of bovine F1-ATPase has been determined previously to 2.8 A, resolution. The enzyme comprises five different subunits in the, stoichiometry alpha 3 beta 3 gamma delta epsilon; the three catalytic beta, subunits alternate with the three alpha subunits around the centrally, located single gamma subunit. To understand more about the catalytic, mechanisms, F1-ATPase was inhibited by reaction with, 4-chloro-7-nitrobenzofurazan (NBD-Cl) and the structure of the inhibited, complex (F1-NBD) determined by X-ray crystallography. RESULTS: In the, structure the three beta subunits adopt a different conformation with, different nucleotide occupancy. ... [(full description)]
About this Structure
1NBM is a [Protein complex] structure of sequences from [Bos taurus] with MG, PO4, ATP and ADP as [ligands]. Active as [Hydrolase], with EC number [3.6.1.34]. Structure known Active Sites: CA1, CA2, CA3, PL1, PL2, PL3, PL4, PL5, PL6 and PL7. Full crystallographic information is available from [OCA].
Reference
Bovine F1-ATPase covalently inhibited with 4-chloro-7-nitrobenzofurazan: the structure provides further support for a rotary catalytic mechanism., Orriss GL, Leslie AG, Braig K, Walker JE, Structure. 1998 Jul 15;6(7):831-7. PMID:9687365
Page seeded by OCA on Tue Oct 30 08:32:39 2007
Categories: Bos taurus | Protein complex | Braig, K. | Leslie, A.G.W. | Orriss, G.L. | Walker, J.E. | ADP | ATP | MG | PO4 | 4-chloro-7-nitrobenzofurazan | Atp synthase | F1-atpase | F1fo atp synthase | Inhibition
