1g3x
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(New page: 200px<br /><applet load="1g3x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g3x, resolution 2.700Å" /> '''INTERCALATION OF AN...)
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Revision as of 22:41, 24 November 2007
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INTERCALATION OF AN 9ACRIDINE-PEPTIDE DRUG IN A DNA DODECAMER
Overview
We present the crystal structure of an acridine drug derivatized at carbon, 9, [N(alpha)-(9-acridinoyl)-tetraarginine], intercalated within the, dodecamer [d(CGCGAATTCGCG)](2). The presence of a lateral chain at the, central carbon 9 atom differentiates this compound from most acridine, drugs hitherto studied, which are usually derivatized at carbon 4. The, DNA:drug interaction we observe differs from that observed in previous, studies, which primarily involves shorter, mainly hexameric sequences, in, two important regards: the acridine intercalates within an AA/TT base, step, rather than within a CG/CG base step; and the binding site is, located at the center of the sequence, rather than at one end of the, duplex. In addition, we observe a novel crystal packing arrangement, with, six dodecamer duplexes and seven hydrated magnesium ions in the asymmetric, unit of a large (66.5 x 68.4 x 77.4 A(3)) unit cell in space group, P2(1)2(1)2(1). The duplexes are organized in layers parallel to the ab, plane, with consecutive layers crossing each other at right angles.
About this Structure
1G3X is a Protein complex structure of sequences from [1] with MG as ligand. Full crystallographic information is available from OCA.
Reference
Intercalation of an acridine-peptide drug in an AA/TT base step in the crystal structure of [d(CGCGAATTCGCG)](2) with six duplexes and seven Mg(2+) ions in the asymmetric unit., Malinina L, Soler-Lopez M, Aymami J, Subirana JA, Biochemistry. 2002 Jul 30;41(30):9341-8. PMID:12135355
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