1sn2
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1sn2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sn2, resolution 1.75Å" /> '''Crystal Structure of...)
Next diff →
Revision as of 22:41, 24 November 2007
|
Crystal Structure of Sea Bream Transthyretin at 1.90A Resolution
Overview
Transthyretin (TTR) is an extracellular transport protein involved in the, distribution of thyroid hormones and vitamin A. So far, TTR has only been, found in vertebrates, of which piscine TTR displays the lowest sequence, identity with human TTR (47%). Human and piscine TTR bind both thyroid, hormones 3,5,3'-triiodo-l-thyronine (T(3)) and, 3,5,3',5'-tetraiodo-l-thyronine (thyroxine, T(4)). Human TTR has higher, affinity for T(4) than T(3), whereas the reverse holds for piscine TTR., X-ray structures of Sparus aurata (sea bream) TTR have been determined as, the apo-protein at 1.75 A resolution and bound to ligands T(3) and T(4), both at 1.9 A resolution. The apo structure is similar to human TTR with, structural changes only at beta-strand D. This strand forms an extended, loop conformation similar to the one in chicken TTR. The piscine TTR.T(4), complex shows the T(4)-binding site to be similar but not identical to, human TTR, whereas the TTR.T(3) complex shows the I3' halogen situated at, the site normally occupied by the hydroxyl group of T(4). The, significantly wider entrance of the hormone-binding channel in sea bream, TTR, in combination with its narrower cavity, provides a structural, explanation for the different binding affinities of human and piscine TTR, to T(3) and T(4).
About this Structure
1SN2 is a Single protein structure of sequence from Sparus aurata with SO4 as ligand. Full crystallographic information is available from OCA.
Reference
High resolution crystal structures of piscine transthyretin reveal different binding modes for triiodothyronine and thyroxine., Eneqvist T, Lundberg E, Karlsson A, Huang S, Santos CR, Power DM, Sauer-Eriksson AE, J Biol Chem. 2004 Jun 18;279(25):26411-6. Epub 2004 Apr 13. PMID:15082720
Page seeded by OCA on Sun Nov 25 00:48:57 2007
