1ofx

From Proteopedia

Revision as of 22:44, 24 November 2007

CRYSTAL STRUCTURE OF AN OKAZAKI FRAGMENT AT 2 ANGSTROMS RESOLUTION

Overview

In DNA replication, Okazaki fragments are formed as double-stranded, intermediates during synthesis of the lagging strand. They are composed of, the growing DNA strand primed by RNA and the template strand. The DNA, oligonucleotide d(GGGTATACGC) and the chimeric RNA-DNA oligonucleotide, r(GCG)d(TATACCC) were combined to form a synthetic Okazaki fragment and, its three-dimensional structure was determined by x-ray crystallography., The fragment adopts an overall A-type conformation with 11 residues per, turn. Although the base-pair geometry, particularly in the central TATA, part, is distorted, there is no evidence for a transition from the A- to, the B-type conformation at the junction between RNA.DNA hybrid and DNA, duplex. The RNA trimer may, therefore, lock the complete fragment in an, A-type conformation.

About this Structure

1OFX is a Protein complex structure of sequences from [1] with SPM as ligand. Full crystallographic information is available from OCA.

Reference

Crystal structure of an Okazaki fragment at 2-A resolution., Egli M, Usman N, Zhang SG, Rich A, Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):534-8. PMID:1370582

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