This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1sqj

From Proteopedia

(Difference between revisions)
Jump to: navigation, search

OCA (Talk | contribs)
(New page: 200px<br /><applet load="1sqj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sqj, resolution 2.20&Aring;" /> '''Crystal Structure An...)
Next diff →

Revision as of 22:53, 24 November 2007

Image:1sqj.gif

1sqj, resolution 2.20Å

Drag the structure with the mouse to rotate

Crystal Structure Analysis of Oligoxyloglucan reducing-end-specific cellobiohydrolase (OXG-RCBH)

Overview

Oligoxyloglucan reducing-end-specific cellobiohydrolase (OXG-RCBH; EC, 3.2.1.150) is an exoglucanase that recognizes the reducing end of, oligoxyloglucan and releases two glucosyl residue segments from the main, chain. The X-ray crystal structure of OXG-RCBH determined at 2.2 A, resolution reveals a unique feature of this enzyme; OXG-RCBH consists of a, tandem repeat of two similar domains, which are both folded into, seven-bladed beta-propeller structures. The sequence alignment of the, propeller blades, based on the structure, indicates that a weak repeat of, the amino acid sequence occurred seven times to construct each domain., There is a cleft that can accommodate the substrate oligosaccharide, between the two domains, which is a putative substrate binding subsite., Mutation of either Asp35 or Asp465, located in the putative catalytic, center, to Asn resulted in a protein with no detectable catalytic, activity, indicating the critical role of these amino acids in catalysis.

About this Structure

1SQJ is a Single protein structure of sequence from Geotrichum sp. m128. Active as Oligoxyloglucan reducing-end-specific cellobiohydrolase, with EC number 3.2.1.150 Full crystallographic information is available from OCA.

Reference

Tandem repeat of a seven-bladed beta-propeller domain in oligoxyloglucan reducing-end-specific cellobiohydrolase., Yaoi K, Kondo H, Noro N, Suzuki M, Tsuda S, Mitsuishi Y, Structure. 2004 Jul;12(7):1209-17. PMID:15242597

Page seeded by OCA on Sun Nov 25 01:00:35 2007

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1sqj"
Personal tools