1kd3
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(New page: 200px<br /><applet load="1kd3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kd3, resolution 1.80Å" /> '''The Crystal Structur...)
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Revision as of 23:01, 24 November 2007
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The Crystal Structure of r(GGUCACAGCCC)2, Thallium form
Overview
Crystals of small RNAs, which regularly diffract to very high resolution, can often be readily obtained. Unfortunately, for some RNAs the, conformations adopted in the crystalline form are different from those, found in solution. For example, short RNAs that form hairpins in solution, virtually never crystallize thus; rather, they form duplexes., Nevertheless, these unintended structures have contributed greatly to the, understanding of RNA structure. In a similar occurrence, the homodimer, r(GGUCACAGCCC)(2) has been crystallized from an 11-mer/12-mer, heteroduplex, r(GGCUGAAGUCCG)/r(GGUCACAGCCC). This surprising phenomenon, was observed under a variety of crystallization conditions. The structure, of the homoduplex was determined from crystals that differed in the, precipitant used and the type of metal present. In all cases, the, resulting homoduplexes contain ten base pairings, of which the central six, are non-canonical pairings. In two of the variants, ordered metal-binding, sites were observed: two equivalent octacoordinate Tl(+) sites in one and, two equivalent nanocoordinate Ba(2+) sites in another.
About this Structure
1KD3 is a Protein complex structure of sequences from [1] with TL as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structures of r(GGUCACAGCCC)2., Kacer V, Scaringe SA, Scarsdale JN, Rife JP, Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):423-32. Epub 2003, Feb 21. PMID:12595698
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