1onm
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(New page: 200px<br /><applet load="1onm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1onm" /> '''Solution Structure of a DNA duplex containin...)
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Revision as of 23:03, 24 November 2007
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Solution Structure of a DNA duplex containing A:G mismatch. d(GCTTCAGTCGT):d(ACGACGGAAGC)
Overview
The efficiency of DNA glycosylases to initiate base excision repair (BER), has been demonstrated to be modulated by the precise sequence context in, which the lesion or mismatch is located. In the case of DNA containing an, A/G mismatch, in which the recognition and excision of adenine from the, mismatch is mediated by the Escherichia coli MutY enzyme, not only does, the local sequence context affect the strength of base stacking, interactions, but it also modulates the syn/anti conformation around the, glycosyl bond of the bases in the mispair. Utilizing prior NMR data to, identify DNA sequence contexts that adopt either an anti/anti or a, syn/anti configuration at an A/G mismatch, we tested the hypothesis that, the initial equilibrium of the mismatched base orientations would modulate, the overall efficiency of glycosyl bond scission. By systematically, varying the sequence context around a central A/G mismatch within a 30-mer, duplex DNA, significant kinetic differences were observed that were, consistent with this hypothesis. Since the relative efficiency of the, kinetics fell into only two groupings, a NMR study was conducted on a DNA, sequence context of unknown syn/anti conformation. These data established, that the relative syn/anti conformation did not correlate with the, excision efficiency, as well as there being a lack of correlation between, kinetics and thermal stability of these DNAs.
About this Structure
1ONM is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Initiation of repair of A/G mismatches is modulated by sequence context., Sanchez AM, Volk DE, Gorenstein DG, Lloyd RS, DNA Repair (Amst). 2003 Aug 12;2(8):863-78. PMID:12893083
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