2cfo
From Proteopedia
(New page: 200px<br /> <applet load="2cfo" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cfo, resolution 2.45Å" /> '''NON-DISCRIMINATING ...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2CFO is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Synechococcus_elongatus Synechococcus elongatus]] with GLU as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.17 6.1.1.17]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CFO OCA]]. | + | 2CFO is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Synechococcus_elongatus Synechococcus elongatus]] with GLU as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Ligase Ligase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.17 6.1.1.17]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CFO OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: protein biosynthesis]] | [[Category: protein biosynthesis]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:35:40 2007'' |
Revision as of 06:30, 30 October 2007
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NON-DISCRIMINATING GLUTAMYL-TRNA SYNTHETASE FROM THERMOSYNECHOCOCCUS ELONGATUS IN COMPLEX WITH GLU
Overview
Error-free protein biosynthesis is dependent on the reliable charging of, each tRNA with its cognate amino acid. Many bacteria, however, lack a, glutaminyl-tRNA synthetase. In these organisms, tRNA(Gln) is initially, mischarged with glutamate by a non-discriminating glutamyl-tRNA synthetase, (ND-GluRS). This enzyme thus charges both tRNA(Glu) and tRNA(Gln) with, glutamate. Discriminating GluRS (D-GluRS), found in some bacteria and all, eukaryotes, exclusively generates Glu-tRNA(Glu). Here we present the first, crystal structure of a non-discriminating GluRS from Thermosynechococcus, elongatus (ND-GluRS(Tel)) in complex with glutamate at a resolution of, 2.45 A. Structurally, the enzyme shares the overall architecture of the, discriminating GluRS from Thermus thermophilus (D-GluRS(Tth)). ... [(full description)]
About this Structure
2CFO is a [Single protein] structure of sequence from [Synechococcus elongatus] with GLU as [ligand]. Active as [Ligase], with EC number [6.1.1.17]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Crystal structure of a non-discriminating glutamyl-tRNA synthetase., Schulze JO, Masoumi A, Nickel D, Jahn M, Jahn D, Schubert WD, Heinz DW, J Mol Biol. 2006 Sep 1;361(5):888-97. Epub 2006 Jul 5. PMID:16876193
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