1gj0
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(New page: 200px<br /><applet load="1gj0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gj0" /> '''NMR STRUCTURE OF AN OLIGONUCLEOTIDE CONTAINI...)
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Revision as of 23:17, 24 November 2007
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NMR STRUCTURE OF AN OLIGONUCLEOTIDE CONTAINING AN ABASIC SITE: BETA ANOMER
Overview
The antitumor antibiotic bleomycin causes two major lesions in the, deoxyribose backbone of DNA: formation of 4'-keto abasic sites and, formation of strand breaks with 3'-phosphoglycolate and 5'-phosphate ends., As a model for the 4'-keto abasic site, we have characterized an abasic, site (X) in d(CCAAAGXACTGGG).d(CCCAGTACTTTGG) by two-dimensional NMR, spectroscopy. A total of 475 NOEs and 101 dihedral angles provided the, restraints for molecular modeling. Four unusual NOEs were observed between, each anomer of the abasic site and the neighboring bases. In addition, four coupling constants for adjacent protons of the deoxyribose of both, the alpha and beta anomers of the abasic site were observed. The modeling, suggests that for both anomers the abasic site is extrahelical, without, significant distortion of the backbone opposite the lesion. The coupling, constants further allowed assignment of an unusual sugar pucker for each, anomer. The unique position of the abasic site in our structural model for, each anomer is discussed in terms of repair of such lesions in vivo.
About this Structure
1GJ0 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of an oligonucleotide containing an abasic site: evidence for an unusual deoxyribose conformation., Hoehn ST, Turner CJ, Stubbe J, Nucleic Acids Res. 2001 Aug 15;29(16):3413-23. PMID:11504879
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