1kj1
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(New page: 200px<br /><applet load="1kj1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kj1, resolution 2.20Å" /> '''MANNOSE-SPECIFIC AGG...)
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Revision as of 23:18, 24 November 2007
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MANNOSE-SPECIFIC AGGLUTININ (LECTIN) FROM GARLIC (ALLIUM SATIVUM) BULBS COMPLEXED WITH ALPHA-D-MANNOSE
Overview
The structure of dimeric garlic lectin was previously determined to an, effective resolution of 2.8A using X-ray intensity data processed by the, XDS package and refined using X-PLOR [Chandra et al. (1999), J. Mol. Biol., 285, 1157--1168]. Repeated attempts to grow better crystals with a view to, improving the definition of the structure did not succeed. The available, raw data were then reprocessed using DENZO. The structure was re-refined, with both X-PLOR and CNS separately using the reprocessed data, which, extended to a resolution of 2.2A. These two sets of refinements and the, two sets using the XDS-processed data afforded an opportunity to compare, the performance of different data-processing and refinement packages when, dealing with data from weakly diffracting crystals. The best results were, obtained when CNS was employed for refinement using data processed by, DENZO. The quality and the resolution of the map and the definition of the, structure improved substantially. In particular, the amino-acid residues, at the variable locations in the sequence, and hence the isolectins, could, be identified with a high degree of confidence. It could be established, that the crystal asymmetric unit contains two identical heterodimers. The, new refined structure also provided a better definition of other finer, structural details.
About this Structure
1KJ1 is a Protein complex structure of sequences from Allium sativum with MAN as ligand. Full crystallographic information is available from OCA.
Reference
Re-refinement using reprocessed data to improve the quality of the structure: a case study involving garlic lectin., Ramachandraiah G, Chandra NR, Surolia A, Vijayan M, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):414-20. Epub 2002, Feb 21. PMID:11856826
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