This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1ous
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1ous" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ous, resolution 1.2Å" /> '''Lecb (PA-LII) calcium...)
Next diff →
Revision as of 23:20, 24 November 2007
|
Lecb (PA-LII) calcium-free
Overview
The crystal structure of Pseudomonas aeruginosa fucose-specific lectin, LecB was determined in its metal-bound and metal-free state as well as in, complex with fucose, mannose and fructopyranose. All three monosaccharides, bind isosterically via direct interactions with two calcium ions as well, as direct hydrogen bonds with several side-chains. The higher affinity for, fucose is explained by the details of the binding site around C6 and O1 of, fucose. In the mannose and fructose complexes, a carboxylate oxygen atom, and one or two hydroxyl groups are partly shielded from solvent upon sugar, binding, preventing them from completely fulfilling their hydrogen bonding, potential. In the fucose complex, no such defects are observed. Instead, C6 makes favourable interactions with a small hydrophobic patch. Upon, demetallization, the C terminus as well as the otherwise rigid, metal-binding loop become more mobile and adopt multiple conformations.
About this Structure
1OUS is a Single protein structure of sequence from Pseudomonas aeruginosa with SO4 as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis of carbohydrate recognition by the lectin LecB from Pseudomonas aeruginosa., Loris R, Tielker D, Jaeger KE, Wyns L, J Mol Biol. 2003 Aug 22;331(4):861-70. PMID:12909014
Page seeded by OCA on Sun Nov 25 01:28:16 2007
