1kl2
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(New page: 200px<br /><applet load="1kl2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1kl2, resolution 2.70Å" /> '''Crystal Structure of...)
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Revision as of 23:24, 24 November 2007
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Crystal Structure of Serine Hydroxymethyltransferase Complexed with Glycine and 5-formyl tetrahydrofolate
Overview
Serine hydroxymethyltransferase (SHMT), a member of the alpha-class of, pyridoxal phosphate-dependent enzymes, catalyzes the reversible conversion, of serine to glycine and tetrahydrofolate to 5,10-methylene, tetrahydrofolate. We present here the crystal structures of the native, enzyme and its complexes with serine, glycine, glycine, and 5-formyl, tetrahydrofolate (FTHF) from Bacillus stearothermophilus. The first, structure of the serine-bound form of SHMT allows identification of, residues involved in serine binding and catalysis. The SHMT-serine complex, does not show any significant conformational change compared with the, native enzyme, contrary to that expected for a conversion from an "open", to "closed" form of the enzyme. However, the ternary complex with FTHF and, glycine shows the reported conformational changes. In contrast to the, Escherichia coli enzyme, this complex shows asymmetric binding of the FTHF, to the two monomers within the dimer in a way similar to the murine SHMT., Comparison of the ternary complex with the native enzyme reveals the, structural basis for the conformational change and asymmetric binding of, FTHF. The four structures presented here correspond to the various, reaction intermediates of the catalytic pathway and provide evidence for a, direct displacement mechanism for the hydroxymethyl transfer rather than a, retroaldol cleavage.
About this Structure
1KL2 is a Protein complex structure of sequences from Geobacillus stearothermophilus with PLP, GLY and FON as ligands. Active as Glycine hydroxymethyltransferase, with EC number 2.1.2.1 Full crystallographic information is available from OCA.
Reference
Crystal structure of binary and ternary complexes of serine hydroxymethyltransferase from Bacillus stearothermophilus: insights into the catalytic mechanism., Trivedi V, Gupta A, Jala VR, Saravanan P, Rao GS, Rao NA, Savithri HS, Subramanya HS, J Biol Chem. 2002 May 10;277(19):17161-9. Epub 2002 Feb 27. PMID:11877399
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