1ovv

From Proteopedia

Revision as of 23:24, 24 November 2007

CRYSTAL STRUCTURE OF FOUR-HELIX BUNDLE MODEL di-Co(II)-DF1-L13A (form II)

Overview

Diffraction data from two non-isomorphous crystals (forms 1 and 2) of an, artificial protein with a four-helix bundle motif, di-Co(II)-DF1-L13A, have been collected using synchrotron radiation. The phase of form 1 has, been assigned using the group and minimal non-isomorphic supergroup, relation between the space group of the previously determined, di-Mn(II)-DF1-L13G structure and the space group of this form. This, unconventional method of solving the phase problem has also been tested, with form 2 using a reverse relation. The structure of the latter form has, been solved using the group and maximal non-isomorphic subgroup relation, with the space group of form 2 of the analogous dimanganese protein. This, application has shown that this phasing method can be used for solving the, protein structures of polymorphic crystals as an alternative to the, molecular-replacement method.

About this Structure

1OVV is a Protein complex structure of sequences from [1] with CO, ACE and NH2 as ligands. Full crystallographic information is available from OCA.

Reference

Phasing protein structures using the group-subgroup relation., Di Costanzo L, Forneris F, Geremia S, Randaccio L, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1435-9. Epub 2003, Jul 23. PMID:12876346

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