1x74
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(New page: 200px<br /><applet load="1x74" size="450" color="white" frame="true" align="right" spinBox="true" caption="1x74, resolution 1.79Å" /> '''Alpha-methylacyl-CoA...)
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Revision as of 23:25, 24 November 2007
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Alpha-methylacyl-CoA racemase from Mycobacterium tuberculosis- mutational and structural characterization of the fold and active site
Overview
Alpha-methylacyl-CoA racemase (Amacr) catalyzes the racemization of, alpha-methyl-branched CoA esters. Sequence comparisons have shown that, this enzyme is a member of the family III CoA transferases. The mammalian, Amacr is involved in bile acid synthesis and branched-chain fatty acid, degradation. In human, mutated variants of Amacr have been shown to be, associated with disease states. Amino acid sequence alignment of Amacrs, and its homologues from various species revealed 26 conserved protic, residues, assumed to be potential candidates as catalytic residues. Amacr, from Mycobacterium tuberculosis (MCR) was taken as a representative of the, racemases. To determine their importance for efficient catalysis, each of, these 26 protic residues of MCR was mutated into an alanine, respectively, and the mutated variants were overexpressed in Escherichia coli. It was, found that four variants (R91A, H126A, D156A, and E241A) were properly, folded but had much decreased catalytic efficiency. Apparently, Arg91, His126, Asp156, and Glu241 are important catalytic residues of MCR. The, importance of these residues for catalysis can be rationalized by the 1.8, A resolution crystal structure of MCR, which shows that the catalytic site, is at the interface between the large and small domain of two different, subunits of the dimeric enzyme. This crystal structure is the first, structure of a complete enzyme of the bile acid synthesis pathway. It, shows that MCR has unique structural features, not seen in the structures, of the sequence related formyl-CoA transferases, suggesting that the, family III CoA transferases can be subdivided in at least two classes, being racemases and CoA transferases.
About this Structure
1X74 is a Single protein structure of sequence from Mycobacterium tuberculosis with PO4 and GOL as ligands. Active as Alpha-methylacyl-CoA racemase, with EC number 5.1.99.4 Full crystallographic information is available from OCA.
Reference
Alpha-methylacyl-CoA racemase from Mycobacterium tuberculosis. Mutational and structural characterization of the active site and the fold., Savolainen K, Bhaumik P, Schmitz W, Kotti TJ, Conzelmann E, Wierenga RK, Hiltunen JK, J Biol Chem. 2005 Apr 1;280(13):12611-20. Epub 2005 Jan 4. PMID:15632186
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