2jhn

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spinqualitylabelsall models 2jhn, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3-METHYLADENINE DNA-GLYCOSYLASE FROM ARCHAEOGLOBUS FULGIDUS

Overview

N(1)-methyladenine (m(1)A) and N(3)-methylcytosine (m(3)C) are major toxic, and mutagenic lesions induced by alkylation in single-stranded DNA. In, bacteria and mammals, m(1)A and m(3)C were recently shown to be repaired, by AlkB-mediated oxidative demethylation, a direct DNA damage reversal, mechanism. No AlkB gene homologues have been identified in Archaea. We, report that m(1)A and m(3)C are repaired by the AfAlkA base excision, repair glycosylase of Archaeoglobus fulgidus, suggesting a different, repair mechanism for these lesions in the third domain of life. In, addition, AfAlkA was found to effect a robust excision of, 1,N(6)-ethenoadenine. We present a high-resolution crystal structure of, AfAlkA, which, together with the characterization of several site-directed, mutants, forms a molecular rationalization for the newly discovered base, excision activity.

About this Structure

2JHN is a Single protein structure of sequence from Archaeoglobus fulgidus with , , , and as ligands. Active as DNA-3-methyladenine glycosylase II, with EC number 3.2.2.21 Known structural/functional Site: . Full crystallographic information is available from OCA.

Reference

Structural basis for enzymatic excision of N1-methyladenine and N3-methylcytosine from DNA., Leiros I, Nabong MP, Grosvik K, Ringvoll J, Haugland GT, Uldal L, Reite K, Olsbu IK, Knaevelsrud I, Moe E, Andersen OA, Birkeland NK, Ruoff P, Klungland A, Bjelland S, EMBO J. 2007 Apr 18;26(8):2206-17. Epub 2007 Mar 29. PMID:17396151

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