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1h19
From Proteopedia
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STRUCTURE OF [E271Q] LEUKOTRIENE A4 HYDROLASE
Overview
Leukotriene A(4) hydrolase/aminopeptidase is a bifunctional zinc, metalloenzyme that converts the fatty acid epoxide leukotriene A(4) into, leukotriene B(4), a potent chemoattractant and immune-modulating lipid, mediator. Recently, the structure of leukotriene A(4) hydrolase revealed, that Glu-271, which belongs to a conserved GXMEN motif in the M1 family of, zinc peptidases, and Gln-136 are located at the active site. Here we, report that mutagenetic replacements of Glu-271, but not Gln-136, abrogate, both catalytic activities of leukotriene A(4) hydrolase. Furthermore, the, 2.1 A crystal structure of [E271Q]leukotriene A(4) hydrolase revealed, minimal conformational changes that could not explain the loss of enzyme, function. We propose that the carboxylate of Glu-271 participates in ... [(full description)]
About this Structure
1H19 is a [Single protein] structure of sequence from [Homo sapiens] with ZN, YB, IMD and ACY as [ligands]. Active as [Leukotriene-A(4) hydrolase], with EC number [3.3.2.6]. Structure known Active Site: ZN1. Full crystallographic information is available from [OCA].
Reference
Leukotriene A4 hydrolase/aminopeptidase. Glutamate 271 is a catalytic residue with specific roles in two distinct enzyme mechanisms., Rudberg PC, Tholander F, Thunnissen MM, Haeggstrom JZ, J Biol Chem. 2002 Jan 11;277(2):1398-404. Epub 2001 Oct 23. PMID:11675384
Page seeded by OCA on Tue Oct 30 15:25:25 2007
