Colicin E7

From Proteopedia

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Colicin E3 is a type of Colicin, a bacteriocin made by E. coli which acts against other nearby E. coli to kill them with its DNase activity; it digests the cell's genome in specific locations, ultimately leading to the death of the cell.

Contents 1 Synthesis and release 2 Mechanism of uptake 3 Killing Activities 4 References

Synthesis and release

After synthesis inside the E. coli cell, the colicin binds its immunity protein, Im7, to its nuclease domain, to prevent the host cell from being killed by its activity. This immunity protein is released only on binding to the outer membrane of the target cell.

Mechanism of uptake

Killing Activities

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Colicin E7 has an endonuclease domain that degrades the DNA of the targeted cell. This structure shows this domain bound to a DNA octamer ^[1].

References

1. ↑ Hsia KC, Chak KF, Liang PH, Cheng YS, Ku WY, Yuan HS. DNA binding and degradation by the HNH protein ColE7. Structure. 2004 Feb;12(2):205-14. PMID:14962381 doi:10.1016/j.str.2004.01.004