1vyb
From Proteopedia
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ENDONUCLEASE DOMAIN OF HUMAN LINE1 ORF2P
Overview
The human L1 endonuclease (L1-EN) is encoded by the non-LTR, retrotransposon LINE-1 (L1). L1 is responsible for more than 1.5 million, retrotransposition events in the history of the human genome, contributing, more than a quarter to human genomic DNA (L1 and Alu elements). L1-EN is, related to the well-understood human DNA repair endonuclease APE1, and its, nicking specificity is a major determinant for retrotransposon integration, site selection. The crystal structure of human L1 endonuclease is the, first of a retrotransposon-encoded protein and a prototype for, retrotransposon-encoded endonucleases involved in target-primed reverse, transcription. Structure-based endonuclease alignments reveal a conserved, threonine in addition to previously identified invariant residues and, suggest that DNA recognition proceeds via the accommodation of an, extrahelical nucleotide within a pocket of the enzyme. The present, analysis will help to refine phylogenetic and functional relationships, among metal-dependent phosphohydrolases and provides a basis for, manipulating non-LTR retrotransposon integration site selection.
About this Structure
1VYB is a Single protein structure of sequence from Homo sapiens with , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structure of the targeting endonuclease of the human LINE-1 retrotransposon., Weichenrieder O, Repanas K, Perrakis A, Structure. 2004 Jun;12(6):975-86. PMID:15274918
Page seeded by OCA on Sun Feb 3 10:17:11 2008
