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2cxa
From Proteopedia
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Crystal structure of Leucyl/phenylalanyl-tRNA protein transferase from Escherichia coli
Overview
Leucyl/phenylalanyl-tRNA-protein transferase (L/F-transferase) is an N-end rule pathway enzyme, which catalyzes the transfer of Leu and Phe from aminoacyl-tRNAs to exposed N-terminal Arg or Lys residues of acceptor proteins. Here, we report the 1.6 A resolution crystal structure of L/F-transferase (JW0868) from Escherichia coli, the first three-dimensional structure of an L/F-transferase. The L/F-transferase adopts a monomeric structure consisting of two domains that form a bilobate molecule. The N-terminal domain forms a small lobe with a novel fold. The large C-terminal domain has a highly conserved fold, which is observed in the GCN5-related N-acetyltransferase (GNAT) family. Most of the conserved residues of L/F-transferase reside in the central cavity, which exists at the interface between the N-terminal and C-terminal domains. A comparison of the structures of L/F-transferase and the bacterial peptidoglycan synthase FemX, indicated a structural homology in the C-terminal domain, and a similar domain interface region. Although the peptidyltransferase function is shared between the two proteins, the enzymatic mechanism would differ. The conserved residues in the central cavity of L/F-transferase suggest that this region is important for the enzyme catalysis.
About this Structure
2CXA is a Single protein structure of sequence from Escherichia coli. Active as Leucyltransferase, with EC number 2.3.2.6 Full crystallographic information is available from OCA.
Reference
The crystal structure of leucyl/phenylalanyl-tRNA-protein transferase from Escherichia coli., Dong X, Kato-Murayama M, Muramatsu T, Mori H, Shirouzu M, Bessho Y, Yokoyama S, Protein Sci. 2007 Mar;16(3):528-34. Epub 2007 Jan 22. PMID:17242373
Page seeded by OCA on Thu Feb 21 16:53:19 2008
Categories: Escherichia coli | Leucyltransferase | Single protein | Bessho, Y. | Kato-Murayama, M. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Shirouzu, M. | Yokoyama, S. | Aminoacyl-trna | National project on protein structural and functional analyses | Nppsfa | Protein degradation | Riken structural genomics/proteomics initiative | Rsgi | Structural genomics | Transferase
