2uye
From Proteopedia
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DOUBLE MUTANT Y110S,F111V DNTR FROM BURKHOLDERIA SP. STRAIN DNT IN COMPLEX WITH THIOCYANATE
Overview
DntR is a bacterial transcription factor that has been isolated from, Burkholderia species that are able to degrade the nitro-aromatic compound, 2,4-dinitrotoluene. We recently solved the X-ray crystal structure of, DntR, which suggested a putative location of an inducer-binding cavity, (IBC). In this study, we constructed mutants of DntR in which residues, lining the proposed IBC were modified in order to identify the structural, elements involved in inducer binding, to modulate the inducer binding, specificity, and to investigate the mechanism of transcriptional, regulation by DntR. The transcriptional activation of the reporter gene, gfp induced by the wild-type and mutant DntRs was monitored by analysing, whole-cell fluorescence using flow-cytometry after addition of a number of, ... [(full description)]
About this Structure
2UYE is a [Single protein] structure of sequence from [Burkholderia cepacia] with SCN and GOL as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
In Vivo and in Vitro Investigation of Transcriptional Regulation by DntR., Lonneborg R, Smirnova I, Dian C, Leonard GA, Brzezinski P, J Mol Biol. 2007 Jul 3;. PMID:17681542
Page seeded by OCA on Tue Oct 30 17:40:24 2007
