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2ivz

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Revision as of 15:12, 30 October 2007 by OCA (Talk | contribs)
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Image:2ivz.gif

2ivz, resolution 2.00Å

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STRUCTURE OF TOLB IN COMPLEX WITH A PEPTIDE OF THE COLICIN E9 T-DOMAIN

Overview

The natively disordered N-terminal 83-aa translocation (T) domain of E, group nuclease colicins recruits OmpF to a colicin-receptor complex in the, outer membrane (OM) as well as TolB in the periplasm of Escherichia coli, the latter triggering translocation of the toxin across the OM. We have, identified the 16-residue TolB binding epitope in the natively disordered, T-domain of the nuclease colicin E9 (ColE9) and solved the crystal, structure of the complex. ColE9 folds into a distorted hairpin within a, canyon of the six-bladed beta-propeller of TolB, using two tryptophans to, bolt the toxin to the canyon floor and numerous intramolecular hydrogen, bonds to stabilize the bound conformation. This mode of binding enables, colicin side chains to hydrogen-bond TolB residues in and around ... [(full description)]

About this Structure

2IVZ is a [Protein complex] structure of sequences from [Escherichia coli] with CA as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Competitive recruitment of the periplasmic translocation portal TolB by a natively disordered domain of colicin E9., Loftus SR, Walker D, Mate MJ, Bonsor DA, James R, Moore GR, Kleanthous C, Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12353-8. Epub 2006 Aug 7. PMID:16894158

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